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cd63 pe  (Miltenyi Biotec)


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    Miltenyi Biotec cd63 pe
    Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti CD81-APC antibody (1:50). I) CTV-stained EVs + human anti <t>CD63-PE</t> antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).
    Cd63 Pe, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd63+pe/bio_rxiv__64898__2026__03__16__712050-94-30-35?v=Miltenyi+Biotec
    Average 94 stars, based on 35 article reviews
    cd63 pe - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "Seminal extracellular vesicles from boar AI doses contain fertility-predictive protein and miRNA cargo and improve sperm physiology"

    Article Title: Seminal extracellular vesicles from boar AI doses contain fertility-predictive protein and miRNA cargo and improve sperm physiology

    Journal: bioRxiv

    doi: 10.64898/2026.03.16.712050

    Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti CD81-APC antibody (1:50). I) CTV-stained EVs + human anti CD63-PE antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).
    Figure Legend Snippet: Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti CD81-APC antibody (1:50). I) CTV-stained EVs + human anti CD63-PE antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).

    Techniques Used: Cytometry, Flow Cytometry, Negative Control, Staining



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    Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti CD81-APC antibody (1:50). I) CTV-stained EVs + human anti <t>CD63-PE</t> antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).
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    Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti CD81-APC antibody (1:50). I) CTV-stained EVs + human anti <t>CD63-PE</t> antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).
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    anti cd63 pe reafinitytm antibodies  (Miltenyi Biotec)
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    MSC-EV characterization. ( A ) Yield of EVs (total amount of EVs per liter of culture medium, mg/L). ( B ) TEM image of the isolated EVs. ( C , D ) Flow cytometry analysis of MSC-EV surface markers, including <t>CD63</t> and CD9. “Dim” and “bright” CD9/CD63 populations were gated based on fluorescence intensity with “dim” indicating low and “bright” indicating high marker expression. ( E ) Analysis of the profiles of growth factors released from AD-MSC-EVs and UC-MSC-EVs by Luminex assay. ( F ) Stability of EVs after multiple freeze‒thaw cycles. The data are presented as mean ± SEM. Significant differences between groups in Figure E and F were analyzed by t-test and ANOVA//Bonferroni, respectively.
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    Image Search Results


    Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti CD81-APC antibody (1:50). I) CTV-stained EVs + human anti CD63-PE antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).

    Journal: bioRxiv

    Article Title: Seminal extracellular vesicles from boar AI doses contain fertility-predictive protein and miRNA cargo and improve sperm physiology

    doi: 10.64898/2026.03.16.712050

    Figure Lengend Snippet: Cytometry plots and histograms of seminal extracellular vesicles (EVs) characterization. A) Flow cytometry beads of know size (200 nm – 2 µm). B) GFP EVs were used to identify EVs population. C) PBS 1X 0.22 μM filtered. D) Negative control of CellTrace Violet (CTV) staining diluted in PBS. E) High fertility (HF) and reduced fertility (RF) unstained EVs. F) HF CTV-stained EVs. G) RF CTV-stained EVs. H) CTV-stained EVs + human anti CD81-APC antibody (1:50). I) CTV-stained EVs + human anti CD63-PE antibody (1:50). J) CTV-stained EVs + human anti Hsp70-FITC antibody (1:50). K) Lysated EVs with Triton 1X + 0.1% SDS. Photodetectors used: Violet Side Scatter – Height (Violet SSC-H), Blue 525-Area (B525-A), Blue 525-Height (B525-H), Violet 450-Height (V450-H), Blue 585-Height (B585-H) and Red 660-Height (R660-H).

    Article Snippet: To immunophenotype the EVs, 25 μL of samples (aproximately 2.0 x 10 9 EVs/mL) were incubated 30 min at 38 °C with conjugated antibodies against specific EVs markers: human anti CD63-PE diluted 1:50 (Ref. 130-118-150, Miltenyi Biotec, Germany), human anti CD81-APC 1:50 (Ref. 130-119-825, Miltenyi Biotec) and human anti Hsp70-FITC 1:50 (Ref. 130-124-700, Miltenyi Biotec).

    Techniques: Cytometry, Flow Cytometry, Negative Control, Staining

    MSC-EV characterization. ( A ) Yield of EVs (total amount of EVs per liter of culture medium, mg/L). ( B ) TEM image of the isolated EVs. ( C , D ) Flow cytometry analysis of MSC-EV surface markers, including CD63 and CD9. “Dim” and “bright” CD9/CD63 populations were gated based on fluorescence intensity with “dim” indicating low and “bright” indicating high marker expression. ( E ) Analysis of the profiles of growth factors released from AD-MSC-EVs and UC-MSC-EVs by Luminex assay. ( F ) Stability of EVs after multiple freeze‒thaw cycles. The data are presented as mean ± SEM. Significant differences between groups in Figure E and F were analyzed by t-test and ANOVA//Bonferroni, respectively.

    Journal: Scientific Reports

    Article Title: Safety evaluation of extracellular vesicles derived from hypoxia primed mesenchymal stem cells of umbilical cord and adipose tissue

    doi: 10.1038/s41598-025-20121-7

    Figure Lengend Snippet: MSC-EV characterization. ( A ) Yield of EVs (total amount of EVs per liter of culture medium, mg/L). ( B ) TEM image of the isolated EVs. ( C , D ) Flow cytometry analysis of MSC-EV surface markers, including CD63 and CD9. “Dim” and “bright” CD9/CD63 populations were gated based on fluorescence intensity with “dim” indicating low and “bright” indicating high marker expression. ( E ) Analysis of the profiles of growth factors released from AD-MSC-EVs and UC-MSC-EVs by Luminex assay. ( F ) Stability of EVs after multiple freeze‒thaw cycles. The data are presented as mean ± SEM. Significant differences between groups in Figure E and F were analyzed by t-test and ANOVA//Bonferroni, respectively.

    Article Snippet: EVs were then stained with human anti-CD9 FITC and anti-CD63 PE REAfinityTM antibodies (Miltenyi Biotec, Germany) and analyzed using a MACSQuant ® Analyzer 10 flow cytometer (Miltenyi Biotec, Germany) and FlowJo software (Beckman Dickson, USA).

    Techniques: Isolation, Flow Cytometry, Fluorescence, Marker, Expressing, Luminex